11/26/2023 0 Comments Gelatin hydrolysis testdoi: 10.3934/microbiol.2021028ĭepartment of Microbiology, Gono Bishwabidalay, Savar, Bangladesh, supported this research project. in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile. Abdul Khalek, Mahmudul Hasan, Farhana Easmin, Nazmi Ara Rumi, Mohammad Shariful Islam. Aoulad Hosen, Fozol Korim Ovi, Harunur Rashid, MD. Furthermore, we suggest selective use of sensitive antimicrobials listed here for therapeutics of cholera outbreak.Ĭitation: Md. Based on these data we recommend using tube-well water instead of river and pond water for drinking purposes. Isolates showed marginal sensitivity towards Tetracycline (33.33%), and Cephalexin (16.67%) and 100% resistance against antibiotics like Vancomycin, Penicillin, Erythromycin, and Nalidixic Acid. All the 12 isolates were sensitive to Gentamicin and ciprofloxacin (100%), while Chloramphenicol (91.67%), Sulfamethoxazole (91.67%), Azithromycin (66.67%) showed high sensitivity. than river and pond water (P 0.05) significantly in 5 different location the sample was collected from. Tube-well water has significantly lower concentration (log CFU/mL) of V. The results showed, out of 45 samples 12 contained V. cholera isolates to determine their antibiotic sensitivity profile. Following isolation antibiotic sensitivity test was performed on each V. cholera and was confirmed by analyzing their biochemical characteristics like Catalase, Oxidase, MR, VP, Indole, Sugar fermentation. Yellow colonies on TCBS agar were screed as V. cholera isolation water samples were first enriched in nutrient broth at 37 ☌ for 16 hours followed by cultivation in selective media TCBS agar at 37 ☌ for 24 hours. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of V. A total of 45 water samples were randomly collected for the isolation and identification of Vibrio spp. cholera in 3 different water sources River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. Therefore, the objective of this research was to isolate V. Serotype O1 of Vibrio cholera is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. Last cholera epidemic has been recorded in Bangladesh between 1992–1993, while few sporadic localized outbreaks have been reported as recent as 2005.
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